Co-immunoprecipitation of Flag-TLR3 or Myc-MSR1 with HCV RNA

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Co-immunoprecipitation of Flag-TLR3 or Myc-MSR1 with HCV RNA.

Co-immunoprecipitation assay of TLR3-Flag or Myc-MSR1 with HCV RNA is used to identify direct interaction of viral RNA with host proteins that recognize viral RNA to initiate interferon (IFN) signaling, a crucial antiviral response of the host cells. Both Toll-like receptor 3 (TLR3) and class-A scavenger receptor type 1 (MSR1) proteins recognize viral double-stranded RNA (dsRNA) which may be re...

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HCV core antigen and HCV-RNA in HIV/HCV co-infected patients with different HCV genotypes

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Class A Scavenger Receptor 1 (MSR1) Restricts Hepatitis C Virus Replication by Mediating Toll-like Receptor 3 Recognition of Viral RNAs Produced in Neighboring Cells

Persistent infections with hepatitis C virus (HCV) may result in life-threatening liver disease, including cirrhosis and cancer, and impose an important burden on human health. Understanding how the virus is capable of achieving persistence in the majority of those infected is thus an important goal. Although HCV has evolved multiple mechanisms to disrupt and block cellular signaling pathways i...

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بررسی مولکولی HCV RNA در اهداکنندگان خون anti-HCV منفی

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ژورنال

عنوان ژورنال: BIO-PROTOCOL

سال: 2014

ISSN: 2331-8325

DOI: 10.21769/bioprotoc.1061